Where are new files?

My problem:

I am starting a new backup on an empty harddrive. This drive ist connected via usb to my desktop computer. Here is Acronis vmProtect 7.0 as a windows agent installed.

After the first run of the backup job, all files from the vm's are in this backup file. I checked this with the file recovery option. Then I created a new file in one of the vm's. After that I startet the same backupjob again. When the backup job was finished I searched for the new file. But I can't find the new file.

Where can I find the new file? Any Idea?